How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments
نویسندگان
چکیده
منابع مشابه
How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.
We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of technical replicates performed and the effect of the volume transferred throughout the dilution se...
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ژورنال
عنوان ژورنال: Biomolecular Detection and Quantification
سال: 2015
ISSN: 2214-7535
DOI: 10.1016/j.bdq.2015.01.005